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Development of polymorphic nuclear microsatellite markers for polyploid and diploid members of the orchid genus Dactylorhiza

Författare:
  • Sofie Olofsson
  • Mikael Hedrén
Publiceringsår: 2007
Språk: Engelska
Sidor: 644-647
Publikation/Tidskrift/Serie: Molecular Ecology Notes
Volym: 7
Nummer: 4
Dokumenttyp: Artikel i tidskrift
Förlag: Wiley-Blackwell

Abstract english

Dactylorhiza traunsteineri is an allotetraploid species that belongs to the large Dactylorhiza incarnata/maculata polyploid complex of the Eurasian genus Dactylorhiza (Orchidaceae). Here, eight polymorphic microsatellite loci were isolated using selective hybridization according to the FIASCO protocol (fast isolation by AFLP of sequences containing repeats) with slight modifications. The number of alleles per locus ranged from two to seven. All loci were possible to amplify in several other species of Dactylorhiza, using the same primers.

Keywords

  • Ecology
  • narrowed-leaved marsh orchid
  • microsatellites
  • cross-species amplification
  • Dactylorhiza
  • polymorphism
  • polyploidy

Other

Published
  • ISSN: 1471-8278
Mikael Hedrén
E-post: mikael [dot] hedren [at] biol [dot] lu [dot] se

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