Meny

Javascript verkar inte påslaget? - Vissa delar av Lunds universitets webbplats fungerar inte optimalt utan javascript, kontrollera din webbläsares inställningar.
Du är här

Identification and characterization of CMP-NeuAc:GalNAc-IgA1 alpha2,6-sialyltransferase in IgA1-producing cells

Författare:
  • Milan Raska
  • Zina Moldoveanu
  • Hitoshi Suzuki
  • Rhubell Brown
  • Rose Kulhavy
  • Judit Andrasi
  • Stacy Hall
  • Huong L Vu
  • Fredric Carlsson
  • Gunnar Lindahl
  • Milan Tomana
  • Bruce A Julian
  • Robert J Wyatt
  • Jiri Mestecky
  • Jan Novak
Publiceringsår: 2007
Språk: Engelska
Sidor: 69-78
Publikation/Tidskrift/Serie: Journal of Molecular Biology
Volym: 369
Nummer: 1
Dokumenttyp: Artikel i tidskrift
Förlag: Elsevier

Abstract english

Glycosylation defects occur in several human diseases. In IgA nephropathy, IgA1 contains O-glycans that are galactose-deficient and consist mostly of core 1 alpha2,6 sialylated N-acetylgalactosamine, a configuration suspected to prevent beta1,3 galactosylation. We confirmed the same aberrancy in IgA1 secreted by the human DAKIKI B cell line. Biochemical assays indicated CMP-NeuAc:GalNAc-IgA1 alpha2,6-sialyltransferase activity in this cell line. However, a candidate enzyme, ST6-GalNAcI, was not transcribed in DAKIKI cells, B cells isolated from blood, or Epstein-Barr virus (EBV)-immortalized IgA1-producing cells from the blood of IgAN patients and healthy controls. Instead, ST6-GalNAcII transcription was detected at a high level. Expression of the ST6-GalNAcII gene and activity of the CMP-NeuAc:GalNAc-IgA1 alpha2,6-sialyltransferase were higher in IgA1-producing cell lines from IgAN patients than in such cells from healthy controls. These data are the first evidence that human cells that lack ST6-GalNAcI can sialylate core 1 GalNAc-Ser/Thr.

Keywords

  • Microbiology in the medical area

Other

Published
  • ISSN: 1089-8638
Fredric Carlsson
E-post: fredric [dot] carlsson [at] biol [dot] lu [dot] se

Universitetslektor

Molekylär cellbiologi

+46 46 222 41 97

B-A315

Sölvegatan 35, Lund

4

Forskargrupp

Mikrobiologigruppen

Doktorander & postdocs

Huvudhandledare